Coach Stephen Keshi has taken four uncapped players to the tournament, which kicks off in Brazil on 15 June.
Keshi has a settled look about his defence but has made a host of changes with Victor Moses and Emmanuel Emenike unavailable.
Joseph Yobo, Peter Odemwingie and Obafemi Martins have been overlooked.
The team travelled out of Namibia on Thursday after their match with the Brave Warriors
They proceeded to Brazil where they have been grouped with Tahiti, Uruguay and World Champions, Spain. The Super Eagles’ first match is against Tahiti on June 17 at the Estadio Mineirao in Belo Horizonte.
Brazil, Japan, Mexico and Italy make up Group A.
Nigeria squad:
Goalkeepers: Chigozie Agbim (Enugu Rangers), Austin Ejide (Hapoel Beer Sheva), Vincent Enyeama (Maccabi Tel Aviv)
Defenders: Efe Ambrose (Celtic), Francis Benjamin (Heartland FC), Elderson Echiejile (Sporting Braga), Azubuike Egwuekwe (Warri Wolves), Solomon Kwambe, Godfrey Oboabona (both Sunshine Stars), Kenneth Omeruo (ADO Den Haag)
Midfielders: Emeka Eze (Enugu Rangers), John Obi Mikel (Chelsea), Fegor Ogude (Valerenga), John Ogu (Academica Coimbra), Ogenyi Onazi (Lazio), Sunday Mba (Enugu Rangers)
Forwards: Joseph Akpala (Werder Bremen), Michael Babatunde (FC Kryvbas), Muhammad Gambo (Kano Pillars), Brown Ideye (Dynamo Kiev), Ahmed Musa (CSKA Moscow), Nnamdi Oduamadi (Varese), Anthony Ujah (FC Cologne).